Screening of Suitable Plant Regeneration Protocols for Several Capsicum spp. through Direct Organogenesis

Peppers (Capsicum spp.) are recalcitrant to in vitro culture regeneration, making the application of vitro-based breeding strategies difficult. We evaluated impact different combinations auxins, cytokinins and micronutrients on induction direct organogenesis cotyledon hypocotyl explants C. annuum, baccatum chinense. found variation regeneration response among species type explant. In this way, average numbers shoots per explant were, respectively, 1.44 0.28 for 4.17 3.20 0.08 0.00 Out six media, best overall results were obtained with medium Pep1, which contained 5 mg/L BAP (6-benzylaminopurine), 0.5 IAA (indole-3-acetic acid) 0.47 CuSO4, followed by a subculture same supplemented 10 AgNO3 (medium Pep1.2). The result Pep1 + Pep1.2 was using explants, 8.87 grown ones greener tissue, while than explants. Our indicate that there is wide Capsicum terms regeneration. suggest synergistic effect copper silver resulted higher rate Explants transferred media elongation, rooting acclimatization. Although acclimatized plantlets chinense, an improvement these latter stages would be desirable high throughput pipeline. This work contributes protocols specific medium, genotype, reaching levels efficiency required genetic transformation gene editing technologies other crops.